Purification and properties of m1-toxin, a specific antagonist of m1 muscarinic receptors
نویسندگان
چکیده
منابع مشابه
Purification and properties of m1-toxin, a specific antagonist of m1 muscarinic receptors.
The venom of the Eastern green mamba from Africa, Dendroaspis angusticeps, was found to block the binding of 3H-quinuclidinyl benzilate to pure m1 and m4 muscarinic ACh receptors expressed in Chinese hamster ovary cells. The principal toxin in the venom with anti-m1 muscarinic activity was purified by gel filtration and reversed-phase HPLC. This toxin has 64 amino acids, a molecular mass of 736...
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Pre-stimulation of Chinese hamster ovary (CHO) cells expressing the human m1-muscarinic receptor (CHO-m1 cells) with a maximally effective concentration of the muscarinic agonist methacholine resulted in desensitization of Ins(1,4,5)P3 accumulation, apparent as a approximately 4-fold shift in the agonist dose-response curve. Agonist-induced desensitization was rapid (detectable by 10 s) and con...
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Muscarinic acetylcholine receptors are extensively involved in cortical cognition and learning-induced or experience-dependent cortical plasticity. The most abundant muscarinic receptor subtype in the cerebral cortex is the M1 receptor, but little is known about its contribution to experience-dependent plasticity of the adult auditory cortex. We have examined the role of the M1 receptor in expe...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1993
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.13-10-04293.1993